rabbit anti mouse cxcl10 antibody  (Proteintech)

Journal: Scientific Reports

Article Title: Blocking PDGF-CC signaling ameliorates multiple sclerosis-like neuroinflammation by inhibiting disruption of the blood–brain barrier

doi: 10.1038/s41598-020-79598-z

Figure Lengend Snippet: Differentially expressed vascular mediators in the BBB transcriptome during the preclinical, progression and remission phase of EAE.

Article Snippet: Cryosections of 12 μm thickness from the different spinal cord segments were obtained and immunofluorescence was performed as follows: Sections were air-dried for 30 min before permeabilization in PBS/0.2% Triton X-100 for 10 min. Staining against rabbit anti-human ADAMTS9, rabbit anti-mouse CXCL10 (Bioss), monoclonal anti-mouse occludin, monoclonal anti-mouse ZO-1, rabbit anti-mouse claudin-5 (all three from Zymed) and rabbit anti-mouse p65 NFκB (RnD Systems), required antigen retrieval with citrate buffer pH 6.0 (Dako) by warming for 20 min in a steamer device (Braun, Germany).

Techniques: Binding Assay, Coagulation

Journal: Frontiers in Microbiology

Article Title: Cocktail of isobavachalcone and curcumin enhance eradication of Staphylococcus aureus biofilm from orthopedic implants by gentamicin and alleviate inflammatory osteolysis

doi: 10.3389/fmicb.2022.958132

Figure Lengend Snippet: Cocktail therapy of Gentamicin with Isobavachalcone and Curcumin alleviate local tissue inflammation, enhance reduction of MDSC M1 Polarization and Eradication of MRSA biofilm in vivo . (A) HE and immunofluorescent staining of distal femur suggest reduction of tissue inflammation and M1 polarization of MDSC, green represents CD11b, a key marker to identify MDSC, while red represents CXCL10, a key marker of activated M1, he black dashed box indicates the approximate location of the implant, and the yellow dashed box range is the area where M1-polarized MDSC in the adjacent implant tissue is observed. (B) Quantification of remaining MRSA in bone with implant. (C) Flow cytometry analysis showed reduction of MDSC in peripheral blood by Gentamicin with ISB or CRM and cocktail therapy. (D) Gating strategy for flow cytometry analysis and representative analysis of frequency of MDSC in peripheral blood. All data were presented as mean ± SD, * represents p < 0.05 and ** represents p < 0.01 when compared to Gentamicin group; # represents p < 0.05 and ## represents p < 0.01 when GEN + ISB + CRM group compared to GEN + ISB/CRM group; GEN, 20 mg/kg/day Gentamycin; ISB, 20 mg/kg/day Isobavachalcone; CRM, 20 mg/kg/day Curcumin.

Article Snippet: The sections were incubated at 4°C overnight with primary antibodies rabbit anti-mouse CD11b (ab184308, 1:500, Abcam, United States) and rabbit anti-mouse CXCL10 (10H11L3, 1:500, Thermo Fisher, United States) for multiplex, respectively; the corresponding secondary antibodies were added onto the sections for 1 h. For immunofluorescence, slides were counterstained with DAPI.

Techniques: In Vivo, Staining, Marker, Flow Cytometry

Journal: Journal of integrative neuroscience

Article Title: Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1.

doi: 10.31083/j.jin2204096

Figure Lengend Snippet: Fig. 1. Expression of Cxcl10 and Nrg1 genes in microarray datasets GSE42828 and GSE93561. (A) Gene expression matrices were

Article Snippet: Following incubation with antibody dilution buffer (Cat no. A1800, Solarbio, Beijing, China), sections were treated with a mixture of the primary rabbit anti-mouse CXCL10 antibody (10937-1-AP, ProteinTech, Rosemont, IL, USA; 1:200) and mouse anti-mouse βIII-Tubulin antibody (sc-80005; Santa Cruz, Dallas, TX, USA; 1:200) [22].

Techniques: Expressing, Microarray, Gene Expression

Journal: Journal of integrative neuroscience

Article Title: Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1.

doi: 10.31083/j.jin2204096

Figure Lengend Snippet: Fig. 3. Effect of CXCL10 and Nrg1 on HT22 cells or NSC34 cells viability. (A–C) Effect of CXCL10 (A) and Nrg1 (B), as well as

Article Snippet: Following incubation with antibody dilution buffer (Cat no. A1800, Solarbio, Beijing, China), sections were treated with a mixture of the primary rabbit anti-mouse CXCL10 antibody (10937-1-AP, ProteinTech, Rosemont, IL, USA; 1:200) and mouse anti-mouse βIII-Tubulin antibody (sc-80005; Santa Cruz, Dallas, TX, USA; 1:200) [22].

Techniques:

Journal: Journal of integrative neuroscience

Article Title: Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1.

doi: 10.31083/j.jin2204096

Figure Lengend Snippet: Fig. 4. Effect of CXCL10 and Nrg1 on HT22 cells and NSC34 cells after scratch injury. (A,C) Effect of CXCL10 and Nrg1 on HT22 cells after scratch injury. n = 4 per group. Scar bar: 200 µm. S.E.M. (***, Nrg1 + CXCL10 group vs. control group, p < 0.001;

Article Snippet: Following incubation with antibody dilution buffer (Cat no. A1800, Solarbio, Beijing, China), sections were treated with a mixture of the primary rabbit anti-mouse CXCL10 antibody (10937-1-AP, ProteinTech, Rosemont, IL, USA; 1:200) and mouse anti-mouse βIII-Tubulin antibody (sc-80005; Santa Cruz, Dallas, TX, USA; 1:200) [22].

Techniques: Control

Journal: Journal of integrative neuroscience

Article Title: Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1.

doi: 10.31083/j.jin2204096

Figure Lengend Snippet: Fig. 5. Effect of CXCL10 and Nrg1 on the molecular expression of HT22 cells. (A) Representative images of pErbB4, pERK1/2, cleaved caspase 9, and cleaved caspase 3. (B–E) Quantitative results of pErbB4, pERK1/2, cleaved caspase 9, and cleaved caspase 3. n

Article Snippet: Following incubation with antibody dilution buffer (Cat no. A1800, Solarbio, Beijing, China), sections were treated with a mixture of the primary rabbit anti-mouse CXCL10 antibody (10937-1-AP, ProteinTech, Rosemont, IL, USA; 1:200) and mouse anti-mouse βIII-Tubulin antibody (sc-80005; Santa Cruz, Dallas, TX, USA; 1:200) [22].

Techniques: Expressing

Journal: Journal of integrative neuroscience

Article Title: Involvement of CXCL10 in Neuronal Damage under the Condition of Spinal Cord Injury and the Potential Therapeutic Effect of Nrg1.

doi: 10.31083/j.jin2204096

Figure Lengend Snippet: Fig. 6. Effect of CXCL10 and Nrg1 on the molecular expression of NSC34 cells. (A) Representative images of pErbB4, pERK1/2, cleaved caspase 9, and cleaved caspase 3. (B–E) Quantitative results of pErbB4, pERK1/2, cleaved caspase 9, and cleaved caspase 3. n

Article Snippet: Following incubation with antibody dilution buffer (Cat no. A1800, Solarbio, Beijing, China), sections were treated with a mixture of the primary rabbit anti-mouse CXCL10 antibody (10937-1-AP, ProteinTech, Rosemont, IL, USA; 1:200) and mouse anti-mouse βIII-Tubulin antibody (sc-80005; Santa Cruz, Dallas, TX, USA; 1:200) [22].

Techniques: Expressing

Journal: Scientific Reports

Article Title: Blocking PDGF-CC signaling ameliorates multiple sclerosis-like neuroinflammation by inhibiting disruption of the blood–brain barrier

doi: 10.1038/s41598-020-79598-z

Figure Lengend Snippet: Differentially expressed vascular mediators in the BBB transcriptome during the preclinical, progression and remission phase of EAE.

Article Snippet: Cryosections of 12 μm thickness from the different spinal cord segments were obtained and immunofluorescence was performed as follows: Sections were air-dried for 30 min before permeabilization in PBS/0.2% Triton X-100 for 10 min. Staining against rabbit anti-human ADAMTS9, rabbit anti-mouse CXCL10 (Bioss), monoclonal anti-mouse occludin, monoclonal anti-mouse ZO-1, rabbit anti-mouse claudin-5 (all three from Zymed) and rabbit anti-mouse p65 NFκB (RnD Systems), required antigen retrieval with citrate buffer pH 6.0 (Dako) by warming for 20 min in a steamer device (Braun, Germany).

Techniques: Binding Assay, Coagulation